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H3K4me1, H3K4me3 and transcription factor binding.

Genome wide relationship between histone H3 lysine 4 mono- and tri-methylation and transcription factor binding.
The work helps clarify relationships between mammalian gene regulation and chromatin modifications by showing for the first time how the histone modifications H3K4me1 and H3K4me3 are associated with transcription factor binding in genome-wide datasets; previously results have been reported only for surrogates of transcription factor binding.
The results clarify uncertainty in the literature about this association for regulatory elements that are distal from gene transcriptional start sites, and start to clarify whether such modification patterns are specific to particular biological systems or are more general.
For the transcription factor STAT1, two results were unexpected. After IFNG stimulation, STAT1 binding was associated with a large fraction (~25%) of 300 thousand active regulatory elements; further, most of the ~70 thousand stimulated binding locations were pre-marked by specific combinations of modified histones, suggesting that some of the regions may be 'poised' for rapid binding in response to signaling.  

Full citation

Robertson AG, Bilenky M, Tam A, Zhao Y, Zeng T, Thiessen N, Cezard T, Fejes AP,
Wederell ED, Cullum R, Euskirchen G, Krzywinski M, Birol I, Snyder M, Hoodless PA, Hirst M, Marra MA, Jones SJ.  Genome Research 2008 Sep. [Epub ahead of print]

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Page last modified Sep 17, 2008