Common regulators between autophagy and cell death
We developed an efficient flow cytometry and RNAi-based
method to identify genes that function in starvation-induced autophagy. We applied this method to 20 known cell death
genes in Drosophila, and identified 6 with an effect on autophagy. Of
particular importance, we are the first to associate an effector caspase Dcp-1
and IAP family protein Bruce with starvation-induced autophagy. Using Drosophila
mutant and transgenic lines, we further show that the caspase is required for
starvation-induced autophagy and is sufficient to induce autophagy in vivo.
This study provides new insights into the molecular mechanisms that
coordinately regulate autophagy and cell death in vivo.
Full citation
Hou YC, Chittaranjan S, Barbosa SG, McCall K, Gorski SM. Effector caspase Dcp-1 and IAP protein Bruce regulate starvation-induced autophagy during Drosophila melanogaster oogenesis. Journal of Cell Biology 2008 182(6):1127-1139.Further details of this publication can be found online at:
http://www.jcb.org/cgi/content/full/182/6/1127
For more information please contact info@bcgsc.ca