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LaneRuler - automated lane tracking software for electrophoresis gels
by Richard Wong — published Apr 25, 2007 — last modified Jun 04, 2010 03:45 PM
This is a short tutorial on the LaneRuler software suite. The core module is a command line c program, whose result can be reviewed and corrected using a java interface. The tutorial will go through the flow of the program, various flags for specifying the format of the gel, and details about all the options available.
Located in Platforms / … / Documentation / LaneRuler Documentation
cDNA gel
by Richard Wong — published Apr 24, 2007 — last modified Jun 04, 2010 03:46 PM
Sample lane tracking result for a cDNA gel.
Located in Platforms / … / Documentation / LaneRuler Documentation
PCR gel
by Richard Wong — published Apr 24, 2007 — last modified Jun 04, 2010 03:46 PM
Sample lane tracking result for a PCR gel.
Located in Platforms / … / Documentation / LaneRuler Documentation
DNA fingerprinting gel
by Richard Wong — published Apr 24, 2007 — last modified Jun 04, 2010 03:46 PM
Sample lane tracking result for a DNA fingerprinting gel.
Located in Platforms / … / Documentation / LaneRuler Documentation
Image PNG image Lane tracking result for a sample DNA fingerprinting gel
by Richard Wong — published Apr 24, 2007 — last modified Jun 04, 2010 03:45 PM
Ran with default parameters. Function call: >findlanes -p <project_directory>
Located in Platforms / … / Documentation / LaneRuler Documentation
Image PNG image Sample lane tracking result for a PCR gel.
by Richard Wong — published Apr 24, 2007 — last modified Jun 04, 2010 03:45 PM
Due to low data density, toneadjustment was turned off. Function call: >findlanes -p <project_directory> -a
Located in Platforms / … / Documentation / LaneRuler Documentation
Image PNG image Sample lane tracking result for a cDNA gel.
by Richard Wong — published Apr 24, 2007 — last modified Jun 04, 2010 03:45 PM
A rotation was required as the gel initially had wells at top edge of the image. Due to low number of marker lanes, we match all peaks in all zones (-i). Tone adjustment was not done due to high data signal (-a). The morphology element size was adjusted since the marker band separation to image width ratio has changed significantly. Function call: >findlanes --numlanes 11 --markerperiod 10 --orientation 1 -a -i --morphelement 150
Located in Platforms / … / Documentation / LaneRuler Documentation
Image TIFF image LaneRuler usage flow chart
by Richard Wong — published Apr 24, 2007 — last modified Jun 04, 2010 03:45 PM
Located in Platforms / … / Documentation / LaneRuler Documentation
Image PNG image Control bar of the LaneRulerUI interface
by Richard Wong — published Apr 24, 2007 — last modified Jun 04, 2010 03:45 PM
Located in Platforms / … / Documentation / LaneRuler Documentation
Image PNG image Panel for parameter specification for the lane adjustment interface
by Richard Wong — published Apr 24, 2007 — last modified Jun 04, 2010 03:45 PM
Located in Platforms / … / Documentation / LaneRuler Documentation
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