ABySS 1.2.1 (Jul 12, 2010)

ABySS

Handle mate pair libraries with reverse-forward orientation as produced by circular, large-fragment libraries. Distance estimates are improved.

For additional information about this project, please visit the overview page .

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Release Notes

State Final release
License BCCA (academic use)

2010-07-12  Shaun Jackman  <sjackman@bcgsc.ca>

    * Release version 1.2.1.
    * Handle reverse-forward oriented mate pair libraries.
    * Improved distance estimates, particularly with large fragment
    libraries.

    abyss-pe:
    * New commands:
    se-contigs: Assemble single-end contigs.
    pe-contigs: Assemble paired-end contigs (default).
    se-sam: Output a gzipped SAM file of the single-end assembly.
    se-bam: Ouptut a BAM file of the single-end assembly.
    pe-dot: Output a Graphviz dot file of the paired-end assembly.
    all: Sam as se-bam pe-contigs pe-dot.
    * Options for one particular library may be specified:
    lib='lib1 lib2' lib2_s=1000 lib2_n=25
    * Input sequence may come from an arbitrary command, which is
    useful to assemble a region of an aligned BAM file:
    in='<(samtools view genome.bam chr10)'

    ABYSS:
    * Bug fix. When reading SAM/BAM files, the quality format
    incorrectly defaulted to ASCII-64, when it should be ASCII-33.

    ABYSS-P:
    * May use the Intel MPI library.

    ParseAligns:
    * Count the number of forward-reverse, reverse-forward and
    forward-forward oriented alignments.

    DistanceEst:
    * Handle reverse-forward oriented mate pair libraries.
    * Improved distance estimates, particularly with large fragment
    libraries.
    * Remove duplicate mate pairs.
    * Print a pretty UTF-8 bar plot of the fragment-size distribution.
    * Multithreaded using OpenMP. The -j, --threads option specifies
    the number of threads to use.
    * Performance improvment.

    Overlap:
    * Handle cases when more than one gap occurs within the mate pair
    fragment size.

    SimpleGraph:
    * Performance improvment.

    MergePaths:
    * Handle the case when a circular sequence is assmembled into a
    single contig.

    abyss-tofastq:
    * New program. Convert qseq, export, SAM and BAM files to FASTA or
    FASTQ format. The files may be compressed with gz, bz2 or xz and
    may be tarred.